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| Registros recuperados: 120 | |
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| Lallias, Delphine; Arzul, Isabelle; Heurtebise, Serge; Ferrand, Sylvie; Chollet, Bruno; Robert, Maeva; Beaumont, Andrew; Boudry, Pierre; Morga, Benjamin; Lapegue, Sylvie. |
| Bonamiosis is a parasitic disease (causative agent: Bonamia ostreae) affecting the European flat oyster Ostrea edulis, responsible for a drastic decline in the aquaculture production of this oyster species. Therefore a selective breeding program for resistance to bonamiosis has been undertaken since 1985 bu Ifremer, leading to the production of several selected oyster families. In the present study, a 6-month cohabitation challenge experiment was performed in order to transmit the disease from wild oysters (injected with the parasite) to two tested families of oysters originating from the selective breeding program. Mortalities were checked daily, and ventricular heart smears were performed on dying or moribund oysters to detect the level of infection to... |
| Tipo: Text |
Palavras-chave: Heart smear; Transmission; Cohabitation experiment; Bonamia ostreae; Ostrea edulis. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/acte-4535.pdf |
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| Harrang, Estelle; Heurtebise, Serge; Faury, Nicole; Robert, Maeva; Arzul, Isabelle; Lapegue, Sylvie. |
| The present study identifies quantitative trait loci (QTLs) in response to an experimental infection with the parasite responsible for bonamiosis, Bonamia ostreae, in two segregating families of the European flat oyster, Ostrea edulis. We first constructed a genetic-linkage map for each studied family and improved the existing genetic-linkage map for the European flat oyster with a set of SNP markers. This latter map now combines the best accuracy and the best estimate of the genome coverage available for an oyster species. Secondly, by comparing the QTLs detected in this study with those previously published for O. edulis in similar experimental conditions, we identified several potential QTLs that were identical between the different families, and also... |
| Tipo: Text |
Palavras-chave: Ostrea edulis; Single Nucleotide Polymorphism; Disease resistance; Genetic and QTL mapping; Candidate genes. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00271/38244/36429.pdf |
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| Arzul, Isabelle; Langlade, Aime; Chollet, Bruno; Robert, Maeva; Ferrand, Sylvie; Omnes, Emmanuelle; Lerond, Sophie; Couraleau, Yann; Joly, Jean-pierre; Francois, Cyrille; Garcia, Celine. |
| Bonamia ostreae is an intracellular protistan parasite affecting flat oysters Ostrea edulis. It can be detected in juveniles but mortalities mainly affect oysters which are more than 2 years old. The parasite is usually observed inside haemocytes and sometimes free, notably in gill epithelia suggesting a parasite release through this organ. However, the infective form and ways of entry and release remain undetermined. Flat oysters incubate their larvae in their pallial cavity for 8–10 days before releasing them into the water column. Flat oysters in Bay of Quiberon in South Brittany (France) are known to be infected with B. ostreae since 1979 and is the most important area in France for O. edulis spat collection. Flat oysters incubating larvae were sampled... |
| Tipo: Text |
Palavras-chave: Bonamia ostreae; Flat oyster; Ostrea edulis; Transmission; Larvae; Parasite life cycle. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00028/13898/12566.pdf |
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| Morga, Benjamin; Renault, Tristan; Faury, Nicole; Chollet, Bruno; Arzul, Isabelle. |
| Bonamia ostreae is a protozoan, affiliated to the order Haplosporidia and to the phylum Cercozoa. This parasite is intracellular and infects haemocytes, cells notably involved in oyster defence mechanisms. Bonamiosis due to the parasite B. ostreae is a disease affecting the flat oyster, Ostrea edulis. The strategies used by protozoan parasites to circumvent host defence mechanisms remain largely unknown in marine bivalve molluscs. In the present work, in vitro experiments were carried out in order to study the interactions between haemocytes from O. edulis and purified parasite. B. ostreae. We monitored cellular and molecular responses of oyster haemocytes by light microscopy, flow cytometry and real-time PCR 1, 2, 4 and 8 h p.i. Light microscopy was used... |
| Tipo: Text |
Palavras-chave: Bonamia ostreae; Protozoan; Ostrea edulis; Haemocytes; Real-time PCR; Flow cytometry; Super oxide dismutase. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00037/14874/18001.pdf |
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| Lopez Flores, L; Suarez Santiago, V; Longet, D; Saulnier, Denis; Chollet, Bruno; Arzul, Isabelle. |
| Bonamia ostreae is a protozoan parasite that infects the European flat oyster Ostrea edulis, causing systemic infections and resulting in massive mortalities in populations of this valuable bivalve species. In this work, we have characterized B. ostreae actin genes and used their sequences for a phylogenetic analysis. Design of different primer sets was necessary to amplify the central coding region of actin genes of B. ostreae. Characterization of the sequences and their amplification in different samples demonstrated the presence of 2 intragenomic actin genes in B. ostreae, without any intron. The phylogenetic analysis placed B. ostreae in a clade with Minchinia tapetis, Minchinia teredinis and Haplosporidium costale as its closest relatives, and... |
| Tipo: Text |
Palavras-chave: Molecular phylogeny; Actin gene; Ostrea edulis parasite; Bonamia ostreae. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3562.pdf |
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| Elgharsalli, Refka; Aloui-bejaoui, Nejla; Sala, Hedi; Chollet, Bruno; Joly, Jean-pierre; Robert, Maeva; Couraleau, Yann; Arzul, Isabelle. |
| Marteilia refringens is a protozoan parasite recognized as a significant pathogen of the European flat oyster Ostrea edulis. The life cycle of this species is still poorly known, although there is evidence of the need for intermediate host(s). In the present study, we have used molecular approaches to identify this parasite in samples of the dwarf oyster Ostrea stentina after reports of massive mortality along the Tunisian coasts. In 2009 we evaluated the status of O. stentina from Monastir and checked if there was an infection with M. refringens, using polymerase chain reaction assays. Of the 103 tested O. stentina, 85 were PCRpositive using a Marteilia genus-specific assay. Additional assays were subsequently carried out on some samples collected in 2010... |
| Tipo: Text |
Palavras-chave: Marteilia refringens; Molecular diagnosis; IGS; ITS; Transmission electron microscopy; Ostrea stentina. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00124/23538/21422.pdf |
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| Lallias, Delphine; Gomez-raya, L.; Haley, C. S.; Arzul, Isabelle; Heurtebise, Serge; Beaumont, A. R.; Boudry, Pierre; Lapegue, Sylvie. |
| We have identified quantitative trait loci (QTL) in the flat oyster (Ostrea edulis) for resistance to Bonamia ostreae, a parasite responsible for the dramatic reduction in the aquaculture of this species. An F-2 family from a cross between a wild oyster and an individual from a family selected for resistance to bonamiosis was cultured with wild oysters injected with the parasite, leading to 20% cumulative mortality. Selective genotyping of 92 out of a total of 550 F-2 progeny (i.e., 46 heavily infected oysters that died and 46 parasite-free oysters that survived) was performed using 20 microsatellites and 34 amplification fragment length polymorphism primer pairs. Both a two-stage testing strategy and QTL interval mapping methods were used. The two-stage... |
| Tipo: Text |
Palavras-chave: Statistical power; Survival analysis; QTL mapping; Disease resistance; Ostrea edulis. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6682.pdf |
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| Comesana, Pilar; Casas, Sandra M.; Cao, Asuncion; Abollo, Elvira; Arzul, Isabelle; Morga, Benjamin; Villalba, Antonio. |
| Farming of the flat oyster Ostrea edulis in Europe is severely constrained by the protozoan Bonamia ostreae. The introduction of the resistant species Crassostrea gigas has been a relief for the farmers, while the pilot programmes to select O. edulis strains resistant to bonamiosis performed in various countries can be seen as a promising strategy to minimise the effects of bonamiosis. However, the physiological bases of this differential susceptibility remain unknown. A search for an explanation of the intra and interspecific differences in oyster susceptibility to bonamiosis was accomplished by comparing some immune parameters among various O. edulis stocks and C gigas. On December 2003, naive and Bonamia-relatively resistant flat oysters from Ireland.... |
| Tipo: Text |
Palavras-chave: Ostrea edulis; Crassostrea gigas; Bonamia ostreae; Haemocyte; Phagocytosis; Respiratory burst; Nitric oxide. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00074/18501/16092.pdf |
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| Morga, Benjamin; Renault, Tristan; Faury, Nicole; Lerond, Sophie; Garcia, Celine; Chollet, Bruno; Joly, Jean-pierre; Lapegue, Sylvie; Harrang, Estelle; Arzul, Isabelle. |
| Bonamiosis due to the parasite Bonamia ostreae has been associated with massive mortality outbreaks in European flat oyster stocks in Europe. As eradication and treatment are not possible, the control of the disease mainly relies on transfer restriction. Moreover, selection has been applied to produce resistant flat oyster families, which present better survival and lower prevalence than non-selected oysters. In order to better understand the mechanisms involved in resistance to bonamiosis, cellular and molecular responses of 2 oyster groups (selected oysters and wild-type oysters) were analyzed in the context of experimental injection and cohabitation infections. Cellular responses including non-specific esterases detection, ROS production and... |
| Tipo: Text |
Palavras-chave: Bonamia ostreae; Protozoan; Ostrea edulis; Resistance; Experimental infection; Haemocytes; Gene expression; Apoptosis. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00405/51665/52213.pdf |
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| Arzul, Isabelle; Chollet, Bruno; Boyer, S.; Bonnet, D.; Gaillard, Juliette; Baldi, Yoann; Robert, Maeva; Joly, Jean-pierre; Garcia, Celine; Bouchoucha, Marc. |
| The paramyxean parasite Marteilia refringens infects several bivalve species including European flat oysters Ostrea edulis and Mediterranean mussels Mytilus galloprovincialis. Sequence polymorphism allowed definition of three parasite types ‘M’, ‘O’ and ‘C’ preferably detected in oysters, mussels and cockles respectively.Transmission of the infection from infected bivalves to copepods Paracartia grani could be experimentally achieved but assays fromcopepods to bivalves failed. In order to contribute to the elucidation of the M. refringens life cycle, the dynamics of the infection was investigated in O. edulis, M. galloprovincialis and zooplankton over one year in Diana lagoon, Corsica (France). Flat oysters appeared non-infected while mussels were infected... |
| Tipo: Text |
Palavras-chave: Bivalves; Marteilia refringens; Copepods; Paracartia latisetosa; Zooplankton; Parasite life cycle. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00165/27635/25768.pdf |
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| Cano, Irene; Ryder, David; Webb, Steve C.; Jones, Brian J.; Brosnahan, Cara L.; Carrasco, Noelia; Bodinier, Barbara; Furones, Dolors; Pretto, Tobia; Carella, Francesca; Chollet, Bruno; Arzul, Isabelle; Cheslett, Deborah; Collins, Evelyn; Lohrmann, Karin B.; Valdivia, Ana L.; Ward, Georgia; Carballal, María J.; Villalba, Antonio; Marigómez, Ionan; Mortensen, Stein; Christison, Kevin; Kevin, Wakeman C.; Bustos, Eduardo; Christie, Lyndsay; Green, Matthew; Feist, Stephen W.. |
| Intracellular microcolonies of bacteria (IMC), in some cases developing large extracellular cysts (bacterial aggregates), infecting primarily gill and digestive gland, have been historically reported in a wide diversity of economically important mollusk species worldwide, sometimes associated with severe lesions and mass mortality events. As an effort to characterize those organisms, traditionally named as Rickettsia or Chlamydia-like organisms, 1950 specimens comprising 22 mollusk species were collected over 10 countries and after histology examination, a selection of 99 samples involving 20 species were subjected to 16S rRNA gene amplicon sequencing. Phylogenetic analysis showed Endozoicomonadaceae sequences in all the mollusk species analyzed.... |
| Tipo: Text |
Palavras-chave: Mollusk; OTU; Endozoicomonas; Chlamydiae; Mycoplasma; Francisella; Lucinoma; Ridgeia. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00658/77032/78322.pdf |
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| Carrasco, N; Arzul, Isabelle; Berthe, Franck; Fernandez Tejedor, M; Durfort, M; Furones, M. |
| Marteilia spp. are paramyxean parasites that affect several bivalve species of economic interest, such as Ostrea edulis and Mytilus galloprovincialis. Certain aspects of Marteilia spp., such as their life cycle and host affinity and infection dynamics, still remain unknown. The 'Delta de l'Ebre' constitutes a natural model for the study of the life cycle of the parasite Marteilia, since uninfected mussels and flat oysters immersed in the bays can become infected. This, along with the geographical and ecological characteristics of the bays, make it a very interesting location to study the Marteilia life cycle. Preliminary results concerning marteiliosis, mainly in mussels, such as prevalence dynamics, infectious periods, host affinity and host intermediate... |
| Tipo: Text |
Palavras-chave: Ebre delta bays; Host specificity; Intermediate host; Mytilus galloprovincialis; Paramyxea; Marteilia. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-3880.pdf |
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| Garcia, Celine; Haond, Christophe; Chollet, Bruno; Nerac, Mirella; Omnes, Emmanuelle; Joly, Jean-pierre; Dubreuil, Christine; Serpin, Delphine; Langlade, Aime; Le Gal, Dominique; Terre-terrillon, Aouregan; Courtois, Olivier; Guichard, Benjamin; Arzul, Isabelle. |
| Background: Microcell parasites are small intracellular protozoans mostly detected in molluscs and can be associated with mortalities. In 2010 and 2011, strong increases in mortality events were reported in different wild beds of the wedge clam Donax trunculus Linnaeus, along the Atlantic coast of France and the presence of potential pathogens, including microcells, was investigated. Methods: Clams collected in different beds showing mortality were examined by histology. Based on histological observations, confirmatory analyses were carried out, including transmission electron microscopy (TEM) and molecular characterization. Results: Histological analyses revealed the presence of small protozoans similar to microcell parasites in different tissues of Donax... |
| Tipo: Text |
Palavras-chave: Mikrocytos veneroides n. sp.; Mikrocytos donaxi n. sp.; Donax trunculus; Mortality; Microcell parasite. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00428/53989/55149.pdf |
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| Nardsi, V.; Arzul, Isabelle; Cargini, D.; Mosca, F.; Calzetta, A.; Traversa, D.; Robert, Maeva; Joly, Jean-pierre; Chollet, Bruno; Renault, Tristan; Tiscar, P. G.. |
| The flat oyster Ostrea edulis L. is widespread along the Italian coasts. In particular, the Manfredonia Gulf (Adriatic Sea) represents an important site where natural beds subsist. Previous monitoring conducted in 1990 by light microscopy and ultrastructural studies revealed the presence of Bonamia-like microcell parasites in some flat oysters: following this observation, a new sampling of O. edulis was carried out at this location in 2007. Of 750 oysters collected, 3 showed the presence of uninucleated microcells (2 to 3 mu m diameter) free or inside the haemocyte cytoplasm by cytology and histopathology. Molecular analysis confirmed that the microcells in 2 oysters were B. exitiosa, whereas in the third oyster the microcells were B. ostreae. Moreover,... |
| Tipo: Text |
Palavras-chave: Bonamia spp.; Ostrea edulis; Molecular analysis; Electron microscopy. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00002/11284/7825.pdf |
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| Batista, Frederico; Arzul, Isabelle; Pepin, Jean-francois; Ruano, Francisco; Friedman, Carolyn S.; Boudry, Pierre; Renault, Tristan. |
| Herpes-like viral infections have been reported in different bivalve mollusc species throughout the world. High mortalities among hatchery-reared larvae and juveniles of different bivalve species have been associated often with such infections. The diagnosis of herpes-like viruses in bivalve molluscs has been performed traditionally by light and transmission electron microscopy. The genome sequencing of one of these viruses, oyster herpesvirus 1 (OsHV-1), allowed the development of DNA-based diagnostic techniques. The polymerase chain reaction (PCR) has been used for the detection of OsHV-1 DNA in bivalve molluscs at different development stages. In addition, the PCR used for detection of OsHV-1 has also allowed the amplification of DNA from an OsHV-1... |
| Tipo: Text |
Palavras-chave: Oyster; Bivalve molluscs; PCR; Detection; OsHV 1; Herpesvirus. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-2322.pdf |
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| Registros recuperados: 120 | |
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